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(Journal Article) |
FEBS letters 583 (7): 1175-82 (2009)
Plant Rho-type (Rop) GTPase-dependent activation of receptor-like cytoplasmic kinases in vitro.
Dulguun Dorjgotov
,
Manuela E Jurca
,
Csilla Fodor-Dunai
,
Attila Szucs
,
Krisztina Otvös
,
Eva Klement
,
Judit Bíró
,
Attila Fehér
ABSTRACT
Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly and specifically activated by GTP-bound Rop GTPases in vitro. Deletion analysis indicated that the residues implicated in the interaction might be distributed on various parts of the kinases. Using a chimaeric Rop GTPase protein, the importance of the Rho-insert region in kinase activation could also be verified. These data strengthen the possibility that RLCKs may serve as Rop GTPase effectors in planta.