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(Journal Article) |
Science (New York, N.Y.) 263 (5150): 1146-9 (1994)
Protein-protein interactions contributing to the specificity of intracellular vesicular trafficking.
N Calakos
,
M K Bennett
,
K E Peterson
,
R H Scheller
ABSTRACT
Intracellular vesicles destined to fuse with the plasma membrane and secrete their contents must have a mechanism for specifically interacting with the appropriate target membrane. Such a mechanism is now suggested by the demonstration of specific interaction between vesicular proteins and plasma membrane proteins. The vesicle-associated membrane proteins (VAMPs) 1 and 2 specifically bind the acceptor membrane proteins syntaxin 1A and 4 but not syntaxin 2 or 3. The binding site is within amino acids 194 to 267 of syntaxin 1A, and the approximate equilibrium dissociation constants is 4.7 x 10(-6) molar. These data suggest a physical basis for the specificity of intracellular vesicular transport.