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(Journal Article) |
Journal of the American Chemical Society 138 (1): 402-7 (2016)
Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11.
Jun-Young Kim
,
Srikanta Sahu
,
Yin-Hoe Yau
,
Xu Wang
,
Susana Geifman Shochat
,
Per Halkjær Nielsen
,
Morten Simonsen Dueholm
,
Daniel E Otzen
,
Jungyeol Lee
,
May Margarette Salido Delos Santos
,
Joey Kuok Hoong Yam
,
Nam-Young Kang
,
Sung-Jin Park
,
Hawyoung Kwon
,
Thomas Seviour
,
Liang Yang
,
Michael Givskov
,
Young-Tae Chang
ABSTRACT
Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.