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(Journal Article) |
Food chemistry 141 (3): 1796-806 (2013)
Simultaneous determination of eighteen steroid hormones in antler velvet by gas chromatography-tandem mass spectrometry.
Chunmei Lu
,
Mingtai Wang
,
Jun Mu
,
Dachuan Han
,
Yuping Bai
,
Hanqi Zhang
ABSTRACT
A method for the simultaneous determination of eighteen steroid hormones, including 17α-ethinylestradiol, 17α-estradiol, 19-nortestosterone, estriol, testosterone, androsterone, 17β-estradiol, estrone, progesterone, 17α-hydroxyprogesterone, medroxyprogesterone, norethisterone acetate, testosterone 17-propionate, medroxyprogesterone-17-acetate, corticosterone, megestrol-17-acetate, chlromadinone-17-acetate and 17β-estradiol-benzoate, in antler velvet was developed by solid-phase extraction (SPE) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Prior to the determination by GC-MS/MS, the analytes were derivatized with heptafluorobutyric acid anhydrides (HFBA). The recoveries were in the range of 62.13-104.00% for eighteen compounds at concentration levels (5-500 μg/L) and relative standard deviations (RSDs) were lower than 17.15%. The detection limits were from 0.2 to 22.3 μg/kg for the steroid hormones. The developed method was rapid, sensitive and successfully applied to the determination of steroid hormones in antler velvet samples collected from three countries.