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(Journal Article) |
Nature reviews. Genetics 10 (1): 57-63 (2009)
RNA-Seq: a revolutionary tool for transcriptomics.
Zhong Wang
,
Mark Gerstein
,
Michael Snyder
ABSTRACT
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.