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Updated in 6/21/2018 8:54:31 AM      Viewed: 496 times      (Journal Article)
Journal of visualized experiments : JoVE (19) (2008)

Morris Water Maze Experiment

Joseph Nunez
ABSTRACT
FROM JOVE.COM:

The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered non-fat milk or non-toxic tempera paint, where they must swim to a hidden escape platform. Because they are in opaque water, the animals cannot see the platform, and cannot rely on scent to find the escape route. Instead, they must rely on external/extra-maze cues. As the animals become more familiar with the task, they are able to find the platform more quickly. Developed by Richard G. Morris in 1984, this paradigm has become one of the "gold standards" of behavioral neuroscience.

DOI: 10.3791/897      ISSN: 1940-087X     
Notes
Setting up the water maze The main component of the water maze set up should be a round pool, about 6 feet in diameter and about 3 feet deep. If you are recording the task with a video camera, make sure all sides of the maze are within the camera’s field of view. Fill up the water maze with tap water, which should be close to 26°C. This may take several hours, so should be done well in advance. Periodically check the water temperature so that it is within one degree of 26°C. Place the escape platform in the center of the pool. During training, it must be exposed, one inch above the water. This teaches the rat that there is a platform, and that it is the way to get out of the water. Later, after the animal is trained and ready for testing, the escape platform will be just below the surface of the water, and will not be visible because the water will be made opaque with milk or non-toxic paint.    Now, the water maze is ready for training the animals. Pre-training for the water maze F Read more...